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Chiaki MURAKAMI
Researchmap
Assistant ProfessorChiba University Institute for Advanced Academic Research / Graduate School of Science and Engineering
Keywords
Phospholipase C, Phosphatidic acid phosphatase, Sphingomyelin synthase-related protein (SMSr), Diacylglycerol, Sphingomyelin synthase, Phosphatidic acid, Bioactive lipids, Diacylglycerol kinase, Lipid biochemistry
Professional Memberships
The Japanese Biochemical Society, Young Researchers Society of Biochemistry, The American Society for Biochemistry and Molecular Biology, The Japanese Conference on the Biochemistry of Lipids
Research Theme
Identification and Characterization of Novel Mammalian Phospholipase Cs
Abstract
Diacylglycerol (DG) is a well-established lipid second messenger that activates protein kinase C (PKC).
In addition to PKC, DG regulates various signal transduction proteins.
In recent years, it has been suggested that the accumulation of saturated fatty acid-containing DG molecular species (SFA-DG) is related to a wide range of physiological and pathological events, including type 2 diabetes and arteriosclerosis.
However, little is known about SFA-DG-generating enzymes.
Recently, we identified a multi-glycerophospholipid phospholipase C hydrolase (MG-PLC) that selectively produces SFA-DG by hydrolyzing glycerophospholipids such as phosphatidylcholine (PC) and phosphatidyl ethanol amine (PE).
Based on the amino acid sequences of MG-PLC, we also identified several types of mammalian PLCs which show PC-specific PLC (PC-PLC) or PE-specific PLC (PE-PLC) activity via homology search.
the molecular entity (the genes and proteins) of mammalian PC-PLC and PE-PLC has not yet been identified since the activity was reported 40 years ago.
This research aims to clarify the physiological significance of novel PLCs.